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Please use the following information to answer the question(s) below. Paulinella chromatophora is one of the few cercozoans that is autotrophic, carrying out aerobic photosynthesis with its two elongated "cyanelles." The cyanelles are contained within vesicles of the host cell, and each is derived from a cyanobacterium, though not the same type of cyanobacterium that gave rise to the chloroplasts of algae and plants. -A crucial photosynthetic gene of the cyanobacterium that gave rise to the cyanelle is called psaE. This gene is present in the nuclear genome of the cercozoan, but is not in the genome of the cyanelle. This is evidence of


A) reciprocal mutations in the cyanelle and nuclear genomes.
B) horizontal gene transfer from bacterium to eukaryotes.
C) genetic recombination involving a protist and an archaean.
D) the origin of photosynthesis in protists.
E) transduction by a phage that infects both prokaryotes and eukaryotes.

F) B) and E)
G) C) and D)

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Please use the following information to answer the question(s) below. Paulinella chromatophora is one of the few cercozoans that is autotrophic, carrying out aerobic photosynthesis with its two elongated "cyanelles." The cyanelles are contained within vesicles of the host cell, and each is derived from a cyanobacterium, though not the same type of cyanobacterium that gave rise to the chloroplasts of algae and plants. -Which of the following represents the true significance of the finding that the cyanelles of P. chromatophora stem from a different type of cyanobacterium than gave rise to chloroplasts?


A) This finding indicates that there is a second evolutionary lineage of photosynthetic eukaryotes.
B) This finding represents the first time that primary endosymbiosis has been directly observed.
C) This finding is the strongest evidence yet for the theory of endosymbiosis.
D) This finding is an example of the phenomenon known as "serial endosymbiosis."
E) This finding is the first evidence that eukaryotic cells do not necessarily digest prokaryotic cells that manage to gain access to their cytoplasm.

F) A) and B)
G) B) and C)

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Please use the following information to answer the question(s) below. Healthy individuals of Paramecium bursaria contain photosynthetic algal endosymbionts of the genus Chlorella. When within their hosts, the algae are referred to as zoochlorellae. In aquaria with light coming from only one side, P. bursaria gathers at the well-lit side, whereas other species of Paramecium gather at the opposite side. The zoochlorellae provide their hosts with glucose and oxygen, and P. bursaria provides its zoochlorellae with protection and motility. P. bursaria can lose its zoochlorellae in two ways: (1) if kept in darkness, the algae will die; and (2) if prey items (mostly bacteria) are absent from its habitat, P. bursaria will digest its zoochlorellae. -Which term most accurately describes the nutritional mode of healthy P. bursaria?


A) photoautotroph
B) photoheterotroph
C) chemoheterotroph
D) chemoautotroph
E) mixotroph

F) A) and D)
G) A) and C)

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E

Of the four supergroups of eukaryotes, which one contains red algae, green algae, and all land plants?


A) SAR
B) Archeplastida
C) Excavata
D) Unikonta

E) A) and B)
F) A) and C)

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The evolution of multicellularity in animals required adaptations that promoted


A) cellular migration and intercellular communication.
B) cellular migration and cellular adhesion.
C) cellular adhesion and intercellular communication.
D) photosynthesis and cellular migration.
E) cellular fusion and regeneration.

F) D) and E)
G) None of the above

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Please use the following information to answer the question(s) below. You are given four test tubes, each containing an unknown protist. Your task is to read the following description and match these four protists to the correct test tube. When red and blue light are shone on the tubes, oxygen bubbles accumulate on the inside of test tubes 1 and 2. Chemical analysis of test tube 1 indicates the presence of a chemical that is toxic to fish and humans. Chemical analysis of test tube 2 indicates the presence of substantial amounts of silica. Microscopic analysis of organisms in test tube 3 reveals the presence of an apicoplast in each. Microscopic analysis of the contents in test tube 4 reveals thousands of cilia on the surface of the organism. -Test tube 4 contains


A) Paramecium.
B) Navicula (diatom) .
C) Pfiesteria (dinoflagellate) .
D) Plasmodium.

E) A) and D)
F) B) and C)

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Which of these observations gives the most support to the endosymbiotic theory for the origin of eukaryotic cells?


A) the existence of structural and molecular differences between the plasma membranes of prokaryotes and the internal membranes of mitochondria and chloroplasts
B) the similarity in size between the cytosolic ribosomes of prokaryotes and the ribosomes within mitochondria and chloroplasts
C) the size disparity between most prokaryotic cells and most eukaryotic cells
D) the observation that some eukaryotic cells lack mitochondria

E) A) and C)
F) B) and C)

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Please use the following information to answer the question(s) below. You are given four test tubes, each containing an unknown protist. Your task is to read the following description and match these four protists to the correct test tube. When red and blue light are shone on the tubes, oxygen bubbles accumulate on the inside of test tubes 1 and 2. Chemical analysis of test tube 1 indicates the presence of a chemical that is toxic to fish and humans. Chemical analysis of test tube 2 indicates the presence of substantial amounts of silica. Microscopic analysis of organisms in test tube 3 reveals the presence of an apicoplast in each. Microscopic analysis of the contents in test tube 4 reveals thousands of cilia on the surface of the organism. -Test tube 1 contains


A) Paramecium.
B) Navicula (diatom) .
C) Pfiesteria (dinoflagellate) .
D) Plasmodium.

E) B) and C)
F) A) and D)

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Please use the following information to answer the question(s) below. Giardia intestinalis is an intestinal parasite of humans and other mammals that causes intestinal ailments in most people. It can infect people when they drink water contaminated with feces containing Giardia cysts. Upon ingestion, each cyst releases two motile cells, called trophozoites. These attach to the small intestine's lining via a ventral adhesive disk. The trophozoites anaerobically metabolize glucose from the host's intestinal contents to produce ATP. Reproduction is completely asexual, occurring by longitudinal binary fission of trophozoites, with each daughter cell receiving two haploid nuclei (n = 5) . A trophozoite will often encyst as it passes into the large intestine by secreting around itself a case that is resistant to cold, heat, and dehydration. -If the mitosomes of Giardia contain no DNA, yet are descendants of what were once free-living organisms, then where are we likely to find the genes that encode their structures, and what accounts for their current location there?


A) plasmids; conjugation
B) plasmids; transformation
C) nucleus; horizontal gene transfer
D) nucleus; S phase

E) All of the above
F) A) and C)

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In a sample of pond water, a new organism is identified with the following characteristics: It consists of 70 cells surrounded by rigid cell walls that join the cells together. Inside each of these identical cells are mitochondria and chloroplasts. Such an organism would most likely be classified as a


A) colonial photosynthetic eukaryote.
B) fungal photosynthetic eukaryote.
C) colonial photosynthetic prokaryote.
D) multicellular photosynthetic prokaryote.
E) colonial eukaryotic fungus.

F) A) and B)
G) A) and C)

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Which of the following pairs of protists and their characteristics are mismatched?


A) apicomplexans-internal parasites
B) mixotrophs-combine photosynthesis and heterotrophic nutrition
C) euglenozoans-unicellular flagellates
D) ciliates-red tide organisms

E) A) and D)
F) All of the above

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D

The chloroplasts of land plants are thought to have been derived according to which evolutionary sequence?


A) cyanobacteria → green algae → land plants
B) cyanobacteria → green algae → fungi → land plants
C) red algae → brown algae → green algae → land plants
D) cyanobacteria → red algae → green algae → land plants

E) A) and D)
F) C) and D)

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Please use the following information to answer the question(s) below. Diatoms are encased in cases (valves) made of translucent hydrated silica whose thickness can vary. The material used to store excess calories can also vary. At certain times, diatoms store excess calories in the form of the liquid polysaccharide laminarin and at other times as oil. The table shows data concerning the density (specific gravity) of various components of diatoms and of their environment. Please use the following information to answer the question(s)  below. Diatoms are encased in cases (valves)  made of translucent hydrated silica whose thickness can vary. The material used to store excess calories can also vary. At certain times, diatoms store excess calories in the form of the liquid polysaccharide laminarin and at other times as oil. The table shows data concerning the density (specific gravity)  of various components of diatoms and of their environment.   -Using dead diatoms to  pump  CO₂ to the seafloor is feasible only if dead diatoms sink quickly. Consequently, application of mineral fertilizers, such as iron, should be most effective at times when diatom valves A)  are thickest, and laminarin is being produced rather than oil. B)  are thickest, and oil is being produced rather than laminarin. C)  are thinnest, and laminarin is being produced rather than oil. D)  are thinnest, and oil is being produced rather than laminarin. -Using dead diatoms to "pump" CO₂ to the seafloor is feasible only if dead diatoms sink quickly. Consequently, application of mineral fertilizers, such as iron, should be most effective at times when diatom valves


A) are thickest, and laminarin is being produced rather than oil.
B) are thickest, and oil is being produced rather than laminarin.
C) are thinnest, and laminarin is being produced rather than oil.
D) are thinnest, and oil is being produced rather than laminarin.

E) All of the above
F) B) and C)

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Please use the following information to answer the question(s) below. Healthy individuals of Paramecium bursaria contain photosynthetic algal endosymbionts of the genus Chlorella. When within their hosts, the algae are referred to as zoochlorellae. In aquaria with light coming from only one side, P. bursaria gathers at the well-lit side, whereas other species of Paramecium gather at the opposite side. The zoochlorellae provide their hosts with glucose and oxygen, and P. bursaria provides its zoochlorellae with protection and motility. P. bursaria can lose its zoochlorellae in two ways: (1) if kept in darkness, the algae will die; and (2) if prey items (mostly bacteria) are absent from its habitat, P. bursaria will digest its zoochlorellae. -When diatoms die, their shells fall to the floor of the ocean or lake that they inhabit and form sediments called diatomaceous earth (DE) . Diatoms can be identified by their shells. Different species of diatoms prefer different water temperatures. What information can be gained if scientists take samples of long-existing DE and identify the diatom species that created it?


A) Scientists can decipher evolutionary lineages between diatom species.
B) Scientists can predict future trends in greenhouse gas emissions.
C) Scientists can learn about past climates.
D) Scientists can better understand how wind currents affect water temperature.

E) A) and B)
F) None of the above

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Multicellular colonies of plant cells adhere to each other primarily by ________, whereas multicellular colonies of animal cells are typically held together by ________.


A) fusion of cellular membranes; cell walls
B) cell walls; fused cellular membranes
C) cell walls; proteins
D) interlocking chloroplasts; interlocking mitochondria
E) interlocking cellular shapes; large, calcified capsules

F) C) and D)
G) A) and C)

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Which of the following statements about dinoflagellates is true?


A) They possess two flagella.
B) All known varieties are autotrophic.
C) Their walls are usually composed of silica plates.
D) Many types lack mitochondria.
E) Their dead cells accumulate on the seafloor and are mined to serve as a filtering material.

F) A) and B)
G) C) and E)

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Please use the following information to answer the question(s) below. Giardia intestinalis is an intestinal parasite of humans and other mammals that causes intestinal ailments in most people. It can infect people when they drink water contaminated with feces containing Giardia cysts. Upon ingestion, each cyst releases two motile cells, called trophozoites. These attach to the small intestine's lining via a ventral adhesive disk. The trophozoites anaerobically metabolize glucose from the host's intestinal contents to produce ATP. Reproduction is completely asexual, occurring by longitudinal binary fission of trophozoites, with each daughter cell receiving two haploid nuclei (n = 5) . A trophozoite will often encyst as it passes into the large intestine by secreting around itself a case that is resistant to cold, heat, and dehydration. -The cysts of Giardia are most analogous to the


A) mitochondria of ancestral diplomonads.
B) nuclei of archaeans.
C) endospores of bacteria.
D) capsids of viruses.

E) None of the above
F) A) and B)

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All protists are


A) unicellular.
B) eukaryotic.
C) symbionts.
D) monophyletic.
E) mixotrophic.

F) A) and C)
G) A) and B)

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Please use the following information to answer the question(s) below. Healthy individuals of Paramecium bursaria contain photosynthetic algal endosymbionts of the genus Chlorella. When within their hosts, the algae are referred to as zoochlorellae. In aquaria with light coming from only one side, P. bursaria gathers at the well-lit side, whereas other species of Paramecium gather at the opposite side. The zoochlorellae provide their hosts with glucose and oxygen, and P. bursaria provides its zoochlorellae with protection and motility. P. bursaria can lose its zoochlorellae in two ways: (1) if kept in darkness, the algae will die; and (2) if prey items (mostly bacteria) are absent from its habitat, P. bursaria will digest its zoochlorellae. -Theoretically, P. bursaria can obtain zoochlorella either vertically (via the asexual reproduction of its mother cell) or horizontally (by ingesting free-living Chlorella from its habitat) . Consider a P. bursaria cell containing zoochlorellae, but whose habitat lacks free-living Chlorella. If this cell subsequently undergoes many generations of asexual reproduction, if all of its daughter cells contain roughly the same number of zoochlorellae as it had originally contained, and if the zoochlorellae are all haploid and identical in appearance, then what is true?


A) The zoochlorellae also reproduced asexually, at an increasing rate over time.
B) The zoochlorellae also reproduced asexually, at a decreasing rate over time.
C) The zoochlorellae also reproduced asexually, at a fairly constant rate over time.
D) The zoochlorellae reproduced sexually, undergoing mitosis and meiosis.

E) C) and D)
F) B) and C)

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C

Why is there controversy surrounding the eukaryotic tree?


A) It suggests that the unikonts are evolutionarily derived from the archeplastids.
B) It suggests that amoebozoans are more closely related (evolutionarily) to red algae than green algae.
C) The tree lacks branches for species that have yet to be classified.
D) The root of the tree is uncertain such that it is unknown which supergroup was the first to diverge from all other eukaryotes.

E) All of the above
F) None of the above

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